Molecular Scale Electrochemistry
Written by Administrator
By linking fluorescence emission to redox state, it is possible, through Total Internal Reflection Fluouescence (TIRF) at electroactive optically-transparent interfaces, to monitor, at the molecular level, reversible electron injection into immobilised proteins. By combining near field proximal probe (AFM) and sensitive far field optical analyses, it is possible to simultaneously map topography and "functional state" in surface anchored biomolecules.
With collaborating teams in Europe we have established protocols where the redox switching characteristics of metalloproteins can be tracked at molecular levels using fluorescence emission. This work is being extended both in mapping sample and surface heterogeneity and monitoring the activity of individual enzyme molecules.
